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KMID : 0903619840250040322
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Journal of the Korean Society for Horticultural Science
1984 Volume.25 No. 4 p.322 ~ p.329
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The Influences of Growth Substances and Carbon Sources on the Development and Induction of sporophytes in Adiantum cuneatum Gametophytes
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Abstract
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This in vitro study was performed to clarify the effect of carbon sources and plant growth regulators on the sporophyte induction and development through the culture of mature sori of Adiantum cuneatum. The results are summarized as follows:
1. Carbon sources are exerted an important role to develop a normal sporeling after germinating of sori. Addition of sucrose at concentration of 2 to 3% to the basal medium was effective on the development of sporelings from gametophytes as compared with glucose or honey bee treatments. But root formation was favored on the medium added honey bee.
2. Picloram at 0.5㎎/ℓ added to the basal medium was promotive on the formation of callus like tissues in gametophytes compared with 2, 4-D treatments. Multiple shoot formation from callus like tissues was also possible, but the growth and leaf development was significantly inhibited and formed shoots were progressively browned and died as passing culture time.
3. Kinetin treatments was not effective on the sporophyte development from the gametophytic tissues. Development of shoots and leaves was inhibited by increasing kinetin concentration on the medium.
4. There was not promotive effects on the development of subcultured young sporelings as affected by various growth substances (tip, IAA, IBA and NAA) and concentrations (0.1∼1.0㎎/ℓ). The addition of BA to the basal medium was inhibited sporeling development as compared with control.
5. Single treatment of 0.1㎎/ℓ Zip or 0.5㎎/ℓ NAA, IAA and IBA was promoted on the sporeling development from the subcultured callus like tissues in vitro.
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